![]() Restriction enzymes are proteins that cleave very specific DNA sequences into fragments at or near to specific recognition sites within molecules known as restriction sites - they are sometimes referred to as 'molecular scissors'. Southern hybridisation/ Southern blottingĪs a clinician, an understanding of the basics behind each technique is key in interpreting results and being able to give simple explanations to patients.Analysis of DNA at the multi-gene level.Analysis of DNA at the nucleotide level. ![]() Molecular analysis techniques can be split into 4 distinct types, each encompassing different techniques and analysis targets: These tests are carried out by biomedical scientists and technicians within labs and their results are key to producing accurate diagnoses that can be used for treatment. Molecular analysis techniques are used in medicine to identify and sequence various organisms and genomes - this allows doctors to come up with diagnoses based on test results. Microarrays (identifies the presence of thousands of DNA sequences from a single genome within a single multi-well plate, allowing for easy comparison between different genomes).Southern hybridisation/Southern blotting (allows for the detection of a specific DNA sequence in a DNA sample). ![]()
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